Biography

Mr. Pudasaini obtained his B.Eng. degree in Mechanical Engineering from Kathmandu University, Nepal and his Ph.D. degree from Nanyang Technological University, Singapore. Prior to joining Nanyang Technological University, he was with Vijay Distillery Pvt. Ltd. in Nepal as a mechanical engineer for a year. He worked as a postdoctoral research associate at Kathmandu Institute of Applied Science (KIAS). Currently, he is working as a postdoctoral research assistant at the University of Glasgow.

Summary

Ph.D. candidate trained in physics and biology, with strong communication skills and ability to work independently or in a team. Special expertise includes:

· Microfluidics/Microfabrication · Experimental Design · Cell Biology

· Image Processing · Numerical Simulation · Data Analysis

Education

Nanyang Technological University, Singapore (2016-2021)

Ph.D., Mechanical and Aerospace Engineering

Kathmandu University, Nepal (2010-2014)

B.E., Mechanical Engineering, GPA 3.66/4.0

Interest

Microfluidics and nanofluidics, Electrokinetic transport phenomena, Electroporation, Thin layer chromatography, Drug analysis

Research Experience

James Watt School of Engineering, University of Glasgow, Scotland (2022-present)

Postdoctoral research assistant

· AST testing using Raman spectroscopy using a microfluidic platform

Kathmandu Institute of Applied Sciences, Nepal (2020-2022)

Postdoctoral researcher

· Developed low cost smartphone operated PCR system to detect antimicrobial resistant bacterial genes

· Developed colorimetric assay to determine ALP enzymes from milk for pasteurisation testing

· Drug quality testing using smartphone application

Nanyang Technological University, Singapore (2016-2020)

Microfluidics based electroporation for inactivation of microorganisms, Ph.D. Student

· Developed microfluidic devices to study electroporation-based inactivation of microorganism

· Analyzed effect of various parameters affecting inactivation process including electric field strength, flowrate, geometry of insulating materials, AC frequency and type of organisms

· Employed numerical method to explain experimental results

· Developed scaled up version of microfluidic device and tested the inactivation performance

Kathmandu University, Nepal (2013-2014)

Completion of the 92 kW Francis turbine test rig, Part time researcher (RENP-PID-437)

· Analyzed erosion effect in all parts of the turbine using CFD

· Strength analysis using fluid-structure interaction

· Analysis of cavitation effect

· Performance testing of turbines experimentally

Kathmandu University, Nepal (2013-2014)

CFD analysis of Pelton Turbine, Final year project

· Scaled down of actual Pelton turbine using IEC 60193 standard

· Obtained pressure distribution, torque and hydraulic efficiency using CFD simulation

· Validation of results using analytical solution

Projects

• Kathmandu University, Nepal

· Automatic water level controller with SMS notification (2013-2014)

· Design and Fabrication of CNC Drilling Machine (2012-2013)

· MATLAB-GUI program for interfacing with 3-axix CNC drilling machine (2012-2013)

Skills

Language: Nepali (Native), English and Hindi

Computer: C, C++, MATLAB, Python, Origin, ImageJ, COMSOL, ANSYS, SolidWorks, Android programming

Lab: Microfabrication, Photolithography, Fluorescence microscopy, Scanning electron microscopy, Thin layer chromatography, Polymerase chain reaction (PCR)